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Effect of aliphatic chain modification of NIR fluorescent dyes using albumin si-RNA in live cells
Project type
Masters project
Date
2017-2018
Location
Mandi, Himachal pradesh
CyG, a renal clearable albumin binding NIR emissive probe has two units i.e. first an albumin binding unit and second a fluorescence reporting unit. The aliphatic chain number controls the hydrophobicity of the dye which regulates the albumin-binding property of the dye. We made different CyG constructs by making aliphatic chain modifications (e.g., ethyl, propyl, hexyl, etc.) and then did live cell imaging using confocal microscopy to see which CyG variant has optimum albumin binding.
Related publication:
Biswas, B., Dey, G., Dogra, S., Mukhopadhyay, A., Chowdhury, S. R., Mondal, P., & Ghosh, S. (2019).
Molecular Scale Optimum Hydrophobicity To Establish an Enhanced Probe-Protein Interaction: Near-Infrared
Imaging of Albumin Biosynthesis Modulation. ACS applied bio materials, 2(8), 3372–3379.
https://doi.org/10.1021/acsabm.9b00362
